Our teams are in regular contact with suppliers and. Processing capacity the sv 96 total rna isolation system is optimized for total rna isolation from cultured cells with a broad spectrum of rna expression levels. The sv 96 total rna isolation system provides a fast, simple technique to purify intact rna from tissue culture cells. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak.
Promega offers genomic dna isolation systems based on sample lysis by detergents and purification. The qiagenkit delivers the highest amount of rna with the highest purity. Automated sv 96 total rna isolation system protocol ep003. Total rna can be purified from 96 samples in less than an hour. The promega wizard sv 96 total rna isolation system provides a highthroughput. The pureyield rna midiprep system allows rapid purification of total rna with undetectable levels of genomic dna contamination without using dnase. The sv total rna isolation system provides a fast and simple technique for preparation of intact total rna from tissues, cultured cells and white blood cells in as little as one hour. Some labs have found it useful to set up an rna work only area that has dedicated labware, pipets, ice buckets, apparatus, etc. Rtpcr, qrtpcr, array analysis, northern blots, nuclease protection assays and rna sequencing.
This technique also allows automation on liquidhandling workstations such as the. Rapid, safe rna purification from cells, bacteria, blood, yeast. The spectrumtm kit showed the highest rna yield and quality among the four, which was tested on roots. Promega sv total rna isolation system sv total rna.
Rna purified with the sv total rna isolation system is suitable for many molecular biology applications, including rtpcr, microarrays and northern blot hybridizations. Dna purification and isolation of genomic dna from. Promega wizard sv 96 total rna isolation system with the instrumentation of the eppendorf epmotion 5075 vac. Comparison of rna isolation methods from insect larvae. Total rna can be purified from 96 samples in less than an hour without centrifugation. There are several methods to isolate rna from insects, including using commercial sv total rna isolation system promega, trizol reagent invitrogen, and the rnaiso reagent takara. Z3101 sv total rna isolation system trial size 10 preps components. Spin column assemblies and elution tubes, dnase i lyophilized, sv rna lysis buffer, sv rna dilution buffer. Like the wizard plus sv dna purification system cat. Efficient isolation of total rna from clostridium without. Pdf quantitative, realtime rtpcr expression using the. Sv 96 total rna isolation system quick protocol, fb075. The sv total rna isolation system provides a fast and simple technique for preparing purified and intact total rna from tissues, cultured cells and white blood cells in as little as one hour, depending on the. The sv 96 total rna isolation system provides a fast, simple technique for the purification of intact, rna from tissue culture cells.
Promega sv total rna isolation system a fast, simple technique for preparation of intact total rna from tissues, cultured cells and white blood cells in as little as one hour. The sv total rna isolation system provides a fast and simple technique for preparing purified and intact total rna from tissues, cultured cells and white blood cells in as little as one hour, depending on the number of samples to be processed. Using this membranebased purification system, up to 60mg of tissue can be processed per purification, depending on tissue type. Sv total rna isolation system promega, for their ability to extract total rna from roots. Rna isolation from cells seeded in 96well plates sv 96 total rna isolation system instructions for use of products z3500 and z3505. Slot blot analysis and ddrtpcr confirm the absence. The system isolates highquality rna from small amounts of.
The sv total rna isolation system has been developed and optimized for total rna isolation from tissues, blood or cultured cells with a broad spectrum of rna expression levels. Promegas new sv total rna isolation system a offers a complete system for the isolation of quality rna from small amounts of starting material. Research note isolation of high quality rna from soil. Total rna extraction products thermo fisher scientific us. Sv 96 total rna isolation system technical bulletin tb294. For more information on downstream applications, see the promega protocols and applications guide 2. Total rna can be purified from 96 samples in less than an hour without. A protocol for simple, quick purification of intact rna from tissue culture cells. Simple spin or vacuumbased method to extract total rna from tissues, cultured cells and white blood cells. Reliaprep mirna cell and tissue miniprep system promega.
Starting with high quality, pure, and intact total rna is critical to many experiments, e. The sv 96 total rna isolation system provides a highthroughput technique to prepare intact rna from tissue and cultured cells. Four methods including three commercial kits rneasy mini kit qiagen, sv total rna isolation system promega. Automated sv 96 total rna isolation system instructions for use of the automated methods with products z3505 and z3500 automated protocol promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516.
Development of a simplified rtpcr without rna isolation. Simple, membranebased method to purify dna from cells, tissues or mouse tails. Simple, 30minute protocol to extract total rna from as few as 100 cells or 0. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Goscript reverse transcription system protocol promega. Four methods including three commercial kits rneasy mini kit qiagen, sv total rna isolation. Isolating rna from insects is becoming increasingly important in molecular entomology. The sv 96 total rna isolation protocol is a multistep procedure that requires that the correct reagents be used in the correct order.
458 526 549 928 1048 1601 290 1271 786 34 538 243 1580 1195 1608 416 662 1151 837 1030 557 584 1107 1540 887 1243 1123 775 754 509 1449 196 905 1465 516 103 308